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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 7
First page : ( 3122) Last page : ( 3128)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00552.1

Methylene Blue Degradation by AgCuO Bimetallic Nanomaterial, Green Synthesized using Cordia sebestena leaves

Ravi Lokesh1, Selvaraj Venkatesh2, Shankar Saranya3, Sundar Ranjitha Dhevi V.3, Segaran Gayathri3, Kumaresan Suganya2, Sadhana Venkatesh4,*

1Department of Botany, St. Joseph's College, Bengaluru

2Sci Wris Life Sciences Pvt. Ltd., Vellore

3School of Biosciences and Technology, VIT University, Vellore

4Chemistry Department Atria Institute of Technology, Bengaluru

*Corresponding Author E-mail: venkysvu9@gmail.com

Online published on 10 August, 2020.


This study reports green synthesis of AgCuO bimetallic nanomaterial from leaf extract of Cordia sebestena and its industrial application as a methylene blue degrading agent. Phytochemical analysis showed that C. sebestena leaves are rich in flavonoids, tannins, saponin, phenols and alkaloids. Aqueous extract of C.sebestena leaves was used as catalyst (1%) and polyethyleneglycol (PEG) was used as capping agent (0.1%) in a reaction mixture containing 1M AgNO3 and 1M CuSO4. The reaction was repeated with AgNO3 and CuSO4 individually for comparison. The synthesized nanomaterial were characterized by SEM, EDX and DLS analysis. Synthesized AgCuO nanomaterial demonstrated sharp needle like clusters ranging 671.6 ± 37.3nm in size. Individual Ag and Cu products, were observed to be in micrometer scale. AgCuO nanomaterial demonstrated most efficient degradation of methylene blue, based on visual and UV spectrum analysis. AgCuO nanomaterial instantly converted the blue colored methylene blue solution into pale green color. Based on gas chromatography mass spectrum (GC-MS) analysis, the mechanism of degradation (chemical attack) was identified, by matching the molecular weight of the fragmented derivatives. AgCuO nanomaterial attacks the Sulphur (S) atom to produce a AgCuS complex, breaking the methylene blue into N, N-dimethylcyclohexa-2,5-dienamine and N, Ndimethylcyclohexa-2,5-diene-1,4-diamine. It was observed that the AgCuO nanomaterials were reusable upto 5times at the tested concentration. The AgCuO Nanomaterial also demonstrated significant antibacterial activity against tested pathogens. These results suggest that the AgCuO Bimetalic Nanomaterial can potentially be applied in treatment of industrial effluents to degrade methylene blue, with added antibacterial effect.



AgCuO Nanomaterial, Bimetallic Nanomaterial, Nanomaterial green synthesis, Methylene blue degradation, Industrial effluent treatment, wastewater treatment.


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