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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 7
First page : ( 3059) Last page : ( 3064)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00543.0

The Sinergy of Isoquercetin and D-Galacturonic acid of Cyclea barbata Miers extract on the Migration and Apoptosis of Neutrophil in Rat Burn Wound

Ismail Dina Dewi S.L.1,2,*, Ratnawati Retty3, Kalim Handono4, Mintaroem Karyono5, Sari Efris Kartika2, Wisnasari Shila2

1Post Graduate Doctoral Program of Medical Science of Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

2Department of Nursing, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

3Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

4Department of Internal Medicine, Faculty of Medicine, University Brawijaya, Malang, Indonesia

5Department of Pathology Anatomy, Faculty of Medicine, University Brawijaya, Malang

*Corresponding Author E-mail: dinasli@ub.ac.id

Online published on 10 August, 2020.


Second-degree burn is one of acute traumas that has complexities in its healing process; one of which is the disruption of neutrophils activity and function as the initial cellular defense during inflammatory phase. Appropriate wound treatment can modulate the quantity of neutrophils in burn wounds. Cyclea barbata Miers (CBM) is a local plant that has a potential to influence inflammatory process. This study was aimed to determine the polyphenol and monosaccharide contents of CBM and the effect of CBM on neutrophils migration and apoptosis. We used 30 adult male Wistar rats as experimental animals, wich were randomly separated in two groups according the eutanized time. Each group divided in 3 groups (n=5 per group). Cyclea barbata Miers’ content was analyzed using liquid chromatography mass spectrometry (LCMS/MS) method, while neutrophils migration and apoptosis were observed using immunofluorescence assay method. The result of LCMS/MS assay showed that CBM contains isoquercetin (93.83ng/mL) and d-galacturonic acid (0.114ng/mL). Further result showed that CBM extract can decrease neutrophil migration and improve neutrophil apoptosis during the inflammatory phase of the burn wound. This ability may be enabled by the anti-inflammatory and antiradical properties of isoquercetin and DGA in Cyclea barbata Miers. Further study is required to confirm this hypothesis, specially addition of comparasion groups that treated by single compound such as isoquercetin and DGA.



Cyclea barbata Miers, Neutrophil, Migration, Apoptosis, Green grass jelly.


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