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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 6
First page : ( 2881) Last page : ( 2885)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00514.4

New stability indicating RP-UFLC method for the determination of Trifluridine – A potent antiviral drug

Yasaswini Rangisetty Spandana, Annapurna Mukthinuthalapati Mathrusri*, Sheela Avuthu Sai

Department of Pharmaceutical Analysis, GITAM Institute of Pharmacy, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh-530045, India

*Corresponding Author E-mail: mmukthin@gitam.edu

Online published on 4 July, 2020.


Trifluridine is an anti-viral drug. A new stability indicating ultrafast liquid chromatographic method has been developed for the assay of Trifluridine in ophthalmic solutions and validated. Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector with C18 Agilent column (250 mm × 4.60 mm i.d. 5μm particle size) and a mobile phase consisting of Acetonitrile: water (50:50, v/v) with flow rate 0.8 ml/min (UV detection at 261 nm) was used for the present study. Trifluridine has shown linearity1-100 μg/mL with linear regression equation y = 66167x + 13368 (R² = 0.9999). Trifluridine was subjected to forced degradation and method was validated as per ICH guidelines.



Trifluridine, RP-UFLC, Validation, Stability Indicating, ICH guidelines.


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