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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 6
First page : ( 2667) Last page : ( 2671)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00474.6

Pharmacognostical Evaluation and HPTLC Analysis of Two Medicinally important Secondary Metabolites in Bauhinia variegata Leaves from Gorakhpur District in Summer Season

Deep Prakash1,*, Dr. Mishra Anurag2, Dr. Verma Neeraj3

1Amity Institute of Pharmacy, Amity University, Lucknow

2Ashoka Institute of Technology and Management, Varanasi

3J K Institute of Technical Education, Lucknow

*Corresponding Author E-mail: pdeep@lko.amity.edu, pdpharma@gmail.com

Online published on 4 July, 2020.



Bauhinia variegata Linn. Which is also known as kachnar belongs to family Fabaceae. It is a medium sized leaf shedding tree generally found in equatorial and temperate region of India. This plant is used in traditional medicine for the treatment of various diseases.


The current study was carried out to find out phytochemical and pharmacognostic standards along with HPTLC analysis of leaves.

Result and Conclusion

The macroscopical studies indicated alternate, green colour, entire margin, linear-lanceolate, rounded and bilobed apex, running parallel venation, smooth and glabrous surface, broad, up to 15 cm long. Physiochemical parameters showed 9.5% total ash, 0.9% acid insoluble ash, 7.65% water-soluble extractive value and loss on drying at 105°C is 7.0%. Preliminary phytochemical screening showed the presence of alkaloid, tannin, steroid, flavonoids, cardiac glycosides, anthraquinone, coumarin, saponins in different extracts. Two flavonoids lupeol and quercetin were notified in HPTLC fingerprinting. The findings of this study could be useful in setting future prospects of this plant.



Bauhinia variegata, HPTLC finger printing, Physico-chemical, Leaf microscopy, Phytochemical constants.


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