Radical scavenging assay and determination Flavonoid and Phenolic total of extract and Fractions of Raghu bark (Dracontomelon dao (Blanco) Merr)
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Raghu bark (Dracontomelon dao (Blanco) Merr) is one of tropical plants that often used by the community to treat skin infectious diseases, skin ulcers, diarrhea, abscesses, inflammations, and tumors.
This research is aimed to test extract and fractions methanol of Dracontomelon dao (Blanco) Merr bark in radical scavenging and to obtain its correlation with the phenolic and flavonoid content.
Dracontomelon dao (Blanco) Merr bark was extracted with methanol and concentrated by evaporator, then added with water and partitioned with n-hexane and ethyl acetate. The methanol extract and the fractions were tested to obtain its radical inhibition power by the DPPH (2, 2-diphenyl-1-picrylhidrazyl) method and determined its phenolic and flavonoid total content.
Methanol extract and fractions of n-hexane, ethyl acetate, and water have strong antiradical. Ethyl acetate is the strongest antiradical inhibition with IC50 = 7.11 ppm and its phenolic and flavonoid content are 75.89 g QE/100 g sample and 58 g GAE/100 g sample, respectively. The correlations of radical inhibition of flavonoid and phenolic content are at 81.6% and 66.51%.
Methanol extract and its fractions of Dracontomelon dao (Blanco) Merr bark have extremely strong radical inhibition. It is recommended to figure out which active compound of Dracontomelon dao (Blanco) Merr bark that has the most responsible for radical inhibition.
Dracontomelon dao (Blanco) Merr bark, DPPH radical, total flavonoid, total phenolic.