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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 5
First page : ( 2228) Last page : ( 2232)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00400.X

Diaphonization of the Ovariectomized Laboratory Animal

Dr. Chitra V., Sharon S Evelyn*

Department of Pharmacology, SRM College of Pharmacy, SRMIST, Kattankulathur-603203, Tamil Nadu, India

*Corresponding Author E-mail: evelynsharonsukumar@gmail.com

Online published on 16 June, 2020.


The main aim of the present study was to present the experience and improvise the protocol for staining the Ovariectomized and non-ovariectomized animal to note the skeletal and cartilage abnormalities. The purpose of the study was to apply the diaphonization technique by double staining with alcian blue and alizarin red. Two female rats were used in the study. One served as control and the other one was ovariectomized. The animals were euthanized followed by removal of the skin and the other major organs. The specimen after clearing of the fatty tissues were fixed in formalin, stained with alcian blue, rehydrated with ethanol, stained with alizarin red and finally preserved in glycerol. The specimens showed good staining of the bone and cartilage. Due to the transparency the skeletal parts were seen clearly. The ovariectomized animal had less staining when compared with the control animal. Calcium is one of the most important mineral in the formation of bones. Having low levels of calcium content might be a major risk for the formation of brittle bones and muscle weakness. Estrogen supports this activity by aiding in calcium absorption. In course of time with this less or decreased deposition of the calcium the skeletal system tend to loss its strength and integrity leading to brittle nature. So it can be concluded that estrogen deficiency might lead to decreased absorption of calcium.



Diaphonization, alcian blue, alizarin red, ovariectomized, bones and cartilage.


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