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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 4
First page : ( 1873) Last page : ( 1877)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00337.6

Effect of delaying Toothbrushing on Enamel surface Roughness during bleaching with different Concentrations of Carbamide peroxide: An In vitro study

Hegde Arjun1, Shetty Preethesh2,*, Bhat Raksha2

1Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal

2A.B. Shetty Memorial Institute of Dental Sciences, Nitte University

*Corresponding Author E-mail: preethesh_shetty@yahoo.co.in

Online published on 30 April, 2020.



To evaluate the effect of delayed tooth brushing with different concentrations of carbamide peroxide on enamel surface roughness.

Materials and Methods

Ninety extracted human permanent premolars were disinfected and the roots were cut at the cemento-enamel junction into 2mm thick enamel slabs measuring 6x4mm were prepared from the middle third of buccal and lingual aspects with the use of double sided diamond discs. One hundred eighty enamel slabs were prepared from ninety permanent extracted human premolars. These one hundred eighty enamel slabs were divided into 3 groups (n=60) based on 3 percentages of carbamide peroxide-15%, 25%, 35%. Every group was again divided into 3 subgroups (n=20)-A, B, C. The subgroup A served as control, subgroup B and subgroup C was based on the time interval of tooth brushing. The initial surface roughness was evaluated using stylus profilometer. For 21days the procedures involving bleaching, brushing and rinsing were continued in all the groups. Once again at the end of 21days, the surface roughness values for the specimens were measured.


Before the study was initiated the means of surface roughness in all the groups were evaluated. The non-parametric Kruskal Wallis test showed significant differences between the groups. The results that the differences in the means of the surface roughness values before and after intervention showed significant differences between the groups under study (p<0.001). The Mann Whitney U test revealed significant differences in the means of surface roughness values of immediately brushed group and the other groups. Group 1 showed least surface roughness were as Group 3 showed increased surface roughness.


Among the different concentrations of bleaching agents available today it is always better and safer to use bleaching agents with lower concentrations. Also. delaying the oral hygiene procedures after bleaching should always be considered to help us in achieving better results.



Bleaching, toothbrushing, carbamide peroxide, stylus profilometer, surface roughness.


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