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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 3
First page : ( 1489) Last page : ( 1494)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00271.1

Simultaneous Determination of Epigallocatechin Gallate, Catechin, and Caffeine from Green Tea Leaves (Camellia sinensis L) Extract by RP-HPLC

Setyawan Eka Indra1,2, Setyowati Erna Prawita2, Rohman Abdul2, Nugroho Akhmad Kharis2,*

1Department of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University, Bali, Indonesia

2Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.

*Corresponding Author E-mail: a.k.nugroho@ugm.ac.id

Online published on 22 April, 2020.

Abstract

This study was aimed to develop sensitive, selective, and simple methods for determining the levels of, catechin, caffeine, and epigallocatechin gallate in green tea leaf extract. The analytical separation was performed using C-18 column and temperature was set on 15 oC. The mobile phase used was ortho-phosphoric acid: water: methanol (0.1: 79.9: 20 v/v/v) pH 3.1 at flow rate 1.0 ml/min. Analyte detection was performed at 280 nm. The analytes were successfully separated with retention time of epigallocatechin gallate, catechin, and caffeine were 16.189, 8.609, and 11.986 minutes, respectively. Linearity of epigallocatechin gallate, catechin, and caffeine were established in the range of 0.5–30 ppm, 0.1–0.6 ppm, and 0.5–5 ppm, respectively. The method was validated as per AOAC guidelines. The level of epigallocatechin gallate, catechin, and caffeine to be founded were 364.73 ± 0.21, 13.86 ± 0.02, and 78.60 ± 0.03 mg/g dry weight, respectively.

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Keywords

Epigallocatechin gallate, Catechin, Caffeine, Green Tea, RP-HPLC.

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