Biochemical Evaluation of Antidiabetic Effect of Kasini ashshifa, a Polyherbal formulation in High Fat Diet Fed-Low Dose STZ Induced Diabetes in Rats
Rao U.S. Mahadeva1,*, Kausar S Akbar2, Janarthanam R. Babu3, Djearamane Sinouvassane4, Kumar S. Suresh5,6,7
1Professor, Head of Biochemistry Unit Faculty of Medicine, Universiti Sultan Zainal Abidin, Kuala, Terengganu, Malaysia.
2President, Al-Kausar Tibb-E-Nabuvi Research Foundation Trust, Vaniyambadi, India.
3Associate Professor, Department of Biotechnology, Thiruvalluvar University, Vellore, India.
4Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia.
5Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.
6Genetics and Regenerative Medicine Research Centre, Universiti Putra Malaysia43400, UPM, Serdang, Selangor, Malaysia.
7Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400, UPM, Selangor, Malaysia.
*Corresponding Author E-mail: firstname.lastname@example.org; email@example.com
Online published on 22 April, 2020.
The study was performed to assess the antidiabetic properties of a polyherbal formulation (Kasini ashshifa) comprising of the thirteen herbals in HFD fed-low dose STZ induced experimental type 2 diabetes (T2D) in rats.
Healthy, male Wistar rats were studied. The rodents were separated into four groups. Group I used as positive control and Group II as negative control. Group III was diabetic rats treated with Kasini ashshifa. Group IV was diabetes rats administered with metformin.
Body weight, food and fluid intake were recorded periodically. OGTT was performed. The biochemical analysis accomplished includes fasting blood glucose, C-peptide, HbA1c, plasma insulin, and urine sugar. Insulin resistance (IR), β-cell function and insulin sensitivity were also manipulated from HOMA-IR and HOMA β-cell function.
The findings of the experiment distinctly indicate that the polyherbal formulation has significantly reduced the readings of fasting blood glucose (FBG) and HbA1c. Kasini ashshifa improves the plasma insulin and C-peptide levels. From the arithmetic data, Kasini ashshifa reduces the IR and ameliorates β-cell function and insulin sensitivity. The antidiabetic efficacy was more noticeable in rats administered with Kasini ashshifa.
The results obtained postulated that the Kasini ashshifa possess significant antidiabetic properties indicating the synergistic effect of herbals.
Kasini ashshifa, Polyherbal, Antidiabetic, insulin resistance, C-peptide.