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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 3
First page : ( 1314) Last page : ( 1318)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00242.5

Analytical Method Development and Validation for biotin from Premixes (solid blend of Multi-Vitamin) by RP-HPLC

Patel Harshita H.1,*, Bhagat Vishwas C.1, Shete Rajkumar V.2, Ravetkar Amit S.3

1Department of Quality Assurance and Techniques, Rajgad Dnyanpeeth's College of Pharmacy, Bhor, Pune, Maharashtra, India

2Department of Pharmacology, Rajgad Dnyanpeeth's College of Pharmacy, Bhor, Pune, Maharashtra, India, rvshete09@gmail.com

3Piramal Enterprises LTD, Mahad Raigad, Maharashtra, India.

*Corresponding Author E-mail: patelharshita558@gmail.com

Online published on 22 April, 2020.


To Develop and Validate Analytical method which is simple, rapid and sensitive for the determination of biotin from premixes (solid blend of multi-vitamin) by RP-HPLC. RP-HPLC analysis was performed using HPLC Agilent Technology 1260 Infinity series HPLC. The chromatographic separation was obtained by using column C18 Thermo scientific ODS Hypersil, 150×4.6, 3 μ, mobile phase used was buffer: acetonitrile in the ratio 91.5: 8.5, Flow rate 1.2 mi/min, wavelength 200nm, Injection volume 100 micro lit, column oven temp. 40°C, run time: 15 min. The retention time of biotin was found to between 10–12 min. Calibration curve was linear with a correlation coefficient of 0.996 over the concentration range of 50–150 ppm and precise with % RSD < 2 for standard and for sample % RSD < 5. The method was found to be specific, linear, precise and accurate. Suitable for the Quality control analysis.



High Pressure Liquid Chromatography (HPLC), Method development, Method validation, Biotin.


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