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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 3
First page : ( 1266) Last page : ( 1270)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00233.4

A Validated Method for The Simultaneous Estimation of Linagliptin and Metformin in Tablet Dosage Forms by RP-HPLC

Sivagami B.1,*, Purushotham A1, Sikdar Pranabesh2, Chandrasekar R.3, Babu M. Niranjan4

1Associate Professor, Department of Pharmaceutical Analysis, Seven Hills College of Pharmacy, Venkataramapuram, Tirupati, Chitoor Dist, 517561, A. P., India

2Associate Professor, Department of Pharmaceutical Chemistry, Seven Hills College of Pharmacy, Venkataramapuram, Tirupati, Chitoor Dist, 517561, A. P., India

3Associate Professor, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkataramapuram, Tirupati, Chitoor Dist, 517561, A. P., India

4Professor, Department of Pharmacognosy, Seven Hills College of Pharmacy, Venkataramapuram, Tirupati, Chitoor Dist, 517561, A. P., India

*Corresponding Author E-mail: sivagamib_27@rediffmail.com

Online published on 22 April, 2020.

Abstract

A simple, Accurate, precise and rapid method was developed for the simultaneous estimation of the Metformin and Linagliptin in Tablet dosage form. Chromatogram was run through Inertsil ODS C18 (250 x 4.6 mm, 5). Mobile phase containing Methanol, Acetonitrile and Water in the ratio of 40: 40: 20 was pumped through column at a flow rate of 1ml/min. Optimized wavelength for Metformin and Linagliptin was 258 nm. Retention time of Metformin and Linagliptin were found to be 2.764 min and 3.162 min %RSD of Metformin and Linagliptin were found to be 0.507 and 0.51 respectively. % Assay was obtained as 99.24% and 99.82% for Metformin and Linagliptin respectively. LOD, LOQ values of Metformin and Linagliptin were 0.08ppm, 0.25ppm and 0.03ppm, 0.08ppm respectively. Regression equation of Metformin was found to be y = 10831x-34273, and for Linagliptin value was found y = 21030x + 31232. Hence the developed method can be successfully employed for the routine analysis of Metformin and Linagliptin in bulk and pharmaceutical dosage forms.

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Keywords

Metformin, Linagliptin, RP-HPLC.

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