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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 3
First page : ( 1179) Last page : ( 1182)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00217.6

Development and Validation of UV Spectroscopic Method for Estimation of Fisetin in Self Nanoemulsifying Drug Delivery System

Kumar Rajan1, Kumar Rakesh1, Khursheed Rubiya1, Kapoor Bhupinder1, Sharma Neha1, Khurana Shelly2, Khurana Navneet1, Singh Sachin Kumar1,*, Vyas Manish1

1School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab-144411, India.

2Department of Pharmacy, Govt. Polytechnic College, Amritsar, Punjab, India

*Corresponding Author E-mail: singhsachin23@gmail.com

Online published on 22 April, 2020.


Fisetin is a polyphenolic flavonoid which has been reported various pharmacological activities as a crude extract and in formulation form. This study was designed to develop and validate a simple and cost effective method for the estimation of fisetin in the liquid self nanoemulsifying drug delivery system (L-SNEDDS). Solutions having different concentration of fisetin were prepared in methanol to develop the calibration curve. The solutions were scanned at 362nm by using UV-spectrophotometer. Similarly, for validation of method recovery of drug was measured at three levels 80%, 100% and 120% of the mid concentration and data was recorded. Further, this method was used to measure the drug loading in L-SNEDDS of fisetin. The data was found to be linear in the range of 4–12μg/mL with regression coefficient of 0.999. The recovery of drug in the range of 95% to 105% indicated the accuracy of the method. The percentage relative standard deviation among all the responses was found to be less than 2% indicating the precision of the method. The LOD and LOQ was found to be 0.28 and 0.86μg/mL respectively. Drug loading in the L-SNEDDS was found to be 93.66%. A simple, accurate and cost-effective method for the estimation of the fisetin in pharmaceutical dosage forms was developed and validated using ultra violet spectroscopy.



Fisetin, Flavonoid, Spectroscopy, Phytochemical, SNEDDS.


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