Anti Bacterial and In Vitro Anti-inflammatory Potential of Boswellia seratta: A Potent Bioactive Compound
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The aim of this study to evaluate the anti bacterial and anti-inflammatory effectiveness of Boswellia serrata standardized extract by in-vitro methods.
The agar diffusion method has been adopted to evaluate the in vitro antibacterial activity of Boswellia serrata against Staphylococcus aureus and Escherchia coli by using Ciprofloxacin as standard drug. The test substance has been screened at different concentrations such as 25, 50, 100, 250, 500 and 1000 μg/ml. The in vitro anti inflammatory activities of Boswellia serrata standardized extract was performed at three different concentrations such as 10μg, 50μg and 100μg by using hyaluronidase enzyme inhibition assay. The absorbance of the final reaction mixture was measured at 600 nm.
The in vitro antibacterial activity of the test sample was evaluated at different concentrations and the diameter of inhibition zones in mm was evaluated. The results revealed that, Boswellia serrata extract at different concentrations provides a promising antimicrobial activity against S. aureus and E.coli. The test compound was screened in a range of 100μg-1000μg in the reaction mixture. The hyaluronidase inhibition potential of test compound was evaluated and calculated as the percentage ratio of the absorbance in presence of the test compound vs. positive control. Standard drug Indomethacin (Indo) was used as reference standard. The result revealed that the maximum (84.847%) hyaluronidase inhibition was observed at 100μl where as standard drug Indomethacin showed (98.21%) inhibition.
The Standardized extract of Boswellia serrata exhibit significant anti bacterial and hyaluronidase inhibition activity in a dose dependent manner.
Boswellia serrata, Anti bacterial, hyaluronidase inhibition, in-vitro methods.