(100.24.118.144)
Users online: 1687    [ij] [ij] [ij] 
Email id
 

Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 915) Last page : ( 922)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00173.0

Spectrophotometric Determination of Carvedilol in Bulk drug and its Formulation by Multivariate Calibration Technique

Nayabaniya Anant*, Seetharaman R**, Lakshmi K.S

Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur-603203, Kancheepuram, Tamil Nadu, India

*Corresponding Author E-mail: anantnayabaniya@gmail.com

**seerampharm@gmail.com

Online published on 9 April, 2020.

Abstract

The determination of Carvedilol in bulk as well as in formulations was done in this study by brining upon a new approach and validating it to be a sensitive, accurate and simple analytical method. The analytical approach was done using a UV spectrophotometer with the application of multivariate calibration. The relationship between concentration and absorbance taken at five different wavelengths including the absorption maxima has been used to develop this multivariate calibration technique. The linear regression equations, residual sum of squares and other mathematical as well as statistical approaches were used for the validation of process according to ICH Q2 (R1) guidelines. The validation parameters that need to be accomplished were precision, accuracy, linearity, and others as mentioned in the guidelines. There was linearity in the plot between the concentration and absorbance in the concentration level of 3–5μg/mL with a good correlation coefficient of R2 = 0.9989. The linear regression equation calculated was: y = 0.1167x + 0.01944. The detection limit and the quantitation limit as calculated were 0.036μg/mL and 0.109 μg/mL respectively. This method has good sensitivity with satisfactory results and can be implemented in routine analysis of bulk drugs and formulations at low cost.

Top

Keywords

Beta-blocker, Carvedilol, Multivariate, calibration, UV Spectrophotometer, Validation.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
597,096,946 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.