The Genoprotective Activity of Aqueous Green Tea extract against Metronidazole and Tinidazole Genotoxic Effect Kahtan Mahmood1, Hassan Ali Faris1, Al-Shawi Nada Naje1, Numan Intesar Tareq2, Bahader Ghaith Abbas3 1Pharmacology And Toxicology Department, College of Pharmacy, University of Baghdad, Baghdad, Iraq 2Pharmacology and Toxicology Department, College of Pharmacy, University of AL-Bayan, Baghdad, Iraq 3Pharmaceutical Chemistry Departments, College of Pharmacy, University of Baghdad, Baghdad, Iraq *Corresponding Author E-mail: ali_1371982@yahoo.com
Online published on 9 April, 2020. Abstract Aim The study designed to evaluate the Geno-protective effect of green tea extract against genotoxicity induced by metronidazole and tinidazole. Methods Thirty-six mice were used, For each experiment, The animals divided into 6 groups: Group I-Negative control administered distilled water; Group II-Healthy mice treated with metronidazole alone, Group III-Healthy mice treated with tinidazole alone; Group IV-Healthy mice administered green tea extract alone Group V-Healthy mice treated with metronidazole, followed by green tea extract administration, Group VI-Healthy mice treated with tinidazole, followed by administration of green tea extract. Results treatment with Tinidazole significantly increase total chromosomal aberration (0.185± 0.0039, 0.167±0.0049) as compared to negative control group (0.116±0.004, 0.103±0.004) in bone marrow and spleen cells respectively. In addition results showed significant change in total chromosomal aberration between the groups (II, IV, V) (0.122±0.012, 0.079±0.005, 0.101±0.0077) (0.121±0.005, 0.067±0.002, 0.087±0.004) in bone marrow cells and spleen cells respectively. In addition, the results shown that there are significant changes in total chromosomal aberration between the groups (III, IV, VI) (0.185±0.0039, 0.079±0.005, 0.133±0.0135) (0.167±0.0049, 0.067±0.002, 0.11±0.012) in bone marrow cells and spleen cells respectively. Conclusion Tinidazole has greater effect than metronidazole on total chromosomal aberration and the aqueous extract of green tea confers Geno-protective effect against the metronidazole or tinidazole induced genotoxicity in mice. Top Keywords Genotoxicity, genoprotection, green tea, metronidazole, tinidazole. Top |