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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 715) Last page : ( 718)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00136.5

An Echo-Friendly and Validated RP-HPLC Method for Simultaneous Determination of Ciprofloxacine HCl and Fluocinolone Acetonide in Bulk and Dosage Forms

Shehada Abdulrahman1, Abdelwahed Wassim2, Sakur Amir Alhaj1,*

1Analytical and Food Chemistry Department-Faculty of Pharmacy, University of Aleppo, Syria

2Pharmaceutics and Pharmaceutical Technology Dept.-Faculty of Pharmacy, University of Aleppo, Syria

*Corresponding Author E-mail: profsakur@gmail.com

Online published on 9 April, 2020.

Abstract

Objective

To develop an echo-friendly, simple, and rapid reversed phase high performance liquid chromatographic (RP-HPLC) method for simultaneous determination of ciprofloxacin (CIP) and fluocinolone acetonide (FluA) as a minor component in bulk and in an otic drop.

Method

CIP and FluA were separated with a C18 column (EC 250/4.6 NUCLEODUR 100-5 C18 ec) at 40˚C oven temperature and flow rate of 1mL/min. The mobile phase consists of 65: 35 methanol: phosphate buffer (pH 3). The buffer contains 0.0125M Heptanesulfonic acid as an ion-pair agent. The analytes were measured with a UV-photo diode array detector at 239 nm.

Results

Chromatograms of high resolution were obtained and the retention times of CIP and FluA were 3.1 and 6.8 min, respectively, and the runtime is only 9 minutes. The method was linear over the concentrations ranging from (20–250)μg/mL for ciprofloxacin and (2.5–600)μg/mL for fluocinolone acetonide, respectively. The assay percentages of ciprofloxacin and fluocinolone acetonide in raw materials were found to be 101.44% and 101.80%, respectively. The developed method was validated by determining its linearity, accuracy, precision, specificity and system suitability.

Conclusion

Developed method showed good reproducibility, and the results were within the accepted limits. It was demonstrated that the method can be successfully applied for the routine determination of CIP and FluA in bulk and pharmaceutical dosage forms.

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Keywords

Ciprofloxacin, Fluocinolone Acetonide, RP-HPLC, Ion-Pair, Antimicrobial Drugs, Echo-Friendly.

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