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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 687) Last page : ( 696)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00132.8

In-vitro Antioxidant, Antimicrobial and Anticancer Activities of Banana leaves (Musa acuminata) and Olive leaves (Olea europaea L.) as by-products

Salama Zeinab A.1, Aboul-Enein Ahmed M.2, Gaafar Alaa A.1,*, Asker Mohsen S.3, Aly Hanan F.4, Ahmed Habiba A.1

1Plant Biochemistry Department, National Research Centre (NRC), 12622, Egypt

2Biochemistry Department Faculty of Agriculture, Cairo University, 12613, Egypt

3Microbial Biotechnology Department, National Research Centre (NRC), 12622, Egypt

4Therapeutic Chemistry Department, National Research Centre (NRC), 12622, Giza, Egypt

*Corresponding Author E-mail: dr.gaafar2308@hotmail.com

Online published on 9 April, 2020.


This study was designed to examine the in-vitro chemical components and their bioactivity as an antioxidant, antimicrobial and anticancer agents for olive leaves (OL) and banana leaves (BL) extracts. Searching for new and cheap sources of important compounds are still not yet discovered. Banana and olive leaves as a source of important and economical compounds are potential for such a purpose. Proximate analysis, Aqueous, methanol, ethanol and acetone extracts of both banana leaves (BL) and olive leaves (OL) were evaluated for their antioxidant, antimicrobial and antitumor agents. Total phenolic (TP), total flavonoids (TF) and total tannins (TT) were determined in their extracts. In addition, HPLC identification for phenolic compounds. Our findings indicate that banana acetone extract possesses a high content of phenolic, flavonoids and tannins. While these compounds were high in methanolic extract of olive leaves. Banana leaves showed high content in phenolic compounds, especially vanillic and ferulic acids. While, protocatechuic, chyrsin, syringic, gallic and ellagic were mostly found in olive leaves. Banana and olive leave acetone extracts found to have considerable antioxidant and antimicrobial activities. Banana leaves acetone extract also presented vigorous activity against breast and liver hepatocellular tumor cell lines. This study concludes that the fruit leaves are a potential source for bioactive metabolites and can be used in the pharmaceutical industry as antimicrobial and antitumor materials. Besides recycling waste to reduce environmental pollution.



Antioxidant, Antimicrobial, Antitumor, Banana, and Olive leaves.


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