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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 604) Last page : ( 608)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00114.6

Stability Indicating RP-HPLC Method for Simultaneous Determination of Atorvastain and Nicotinic acid from Tablet Dosage Form

Dhadve Atish Z.1,*, Dashetwar Anupama R.2, Kardile Dipak P.3, Shete Rajkumar V.4

1Department of Quality Assurance Techniques, Rajgad Dnyanpeeth's College of Pharmacy Bhor, Dist-Pune, 412206, Maharashtra, India

2Department of Quality Assurance Technique, Rajgad Dnyanpeeth's College of Pharmacy Bhor, Dist-Pune, 412206, Maharashtra, India

3Department of Pharmaceutical Chemistry, Rajgad Dnyanpeeth's College of Pharmacy Bhor, Dist-Pune, 412206, Maharashtra, India

4Department of Pharmacology, Rajgad Dnyanpeeth's College of Pharmacy Bhor, Dist-Pune, 412206, Maharashtra, India

*Corresponding Author E-mail: atishdhadve@gmail.com

Online published on 9 April, 2020.

Abstract

To develop simple, selective, specific, accurate and rapid Stability Indicating RP-HPLC method for simultaneous determination of Atorvastatin and nicotinic acid from tablet dosage form. The chromatographic separations of two lipid lowering drug were carried out by using Thermo ODS (5μm. 150x4.6 mm i.d column) maintained at ambient temp (25°C) throughout the analysis. The drug was separated by using mobile phase consist of Acetonitrile and 0.1% acetic acid (70: 30) by using isocratic elution mode separation at the flow rate of 1ml/min and detection wavelength of 245nm by using UV detector. The method were found to be linear over the concentration range of 2–10μg/ml and 75–375μg/ml with correlation coefficient0.9999 and 0.9960 for the atorvastatin and Nicotinic acid respectively. The retention time of Atorvastatin calcium and Nicotinic acid were 1.539 and 2.475 min. respectively The developed method was validated as per ICH guideline and successfully applied for determination of Atorvastatin calcium and Nicotinic acid in tablet dosage form.

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Keywords

Atorvastatin calcium, Nicotinic acid, Stability indicating, RP-HPLC, Validation.

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