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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 583) Last page : ( 588)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00110.9

Gradient RP-HPLC Method development for simultaneous estimation of Dextromethorphan hydrobromide, Phenylephrine hydrochloride, and Triprolidine hydrochloride in Liquid Dosage Form

Baghel Uttam Singh1,*, Sharma Himani2, Chouhan Anamika3, Sharma Abhay3, Mukim Mohammad3, Singh Deeksha4,**

1Department of Pharmacy, University of Kota, MBS Marg, Swami Vivekanand Nagar, Kota, 324005, Rajasthan, India

2Department of Pharmaceutical Analysis, ASBASJSM College of Pharmacy, BELA (Ropar), Punjab-140111, India

3Kota College of Pharmacy, SP-1, Industrial Area, Jhalawar Road, Ranpur, Kota, 325003, Rajasthan, India

4ESI Hospital, Jhalawar Road, Vigyan Nagar, Kota, Rajasthan, India

*Corresponding Author E-mail: drusb1985@yahoo.com


Online published on 9 April, 2020.


A novel, sensitive, and specific gradient RP-HPLC method with UV detection is proposed for the simultaneous quantification of multicomponent liquid dosage form. The separation of the three drugs was achieved using a C18 column (250 mm×4.0 mm, 5 μ) as a stationary phase. No preliminary extraction procedure is required for liquid formulations and a very simple extraction procedure is required for tablets and creams. A mixture of two mobile phases was used with gradient time programing at a flow rate of 1 ml/min. Detection was performed by using PDA detector at 270 nm. Phenylephrine HCl (RT=7.705 min), triprolidine HCl (RT=26.510 min) and dextromethorphan HBr (RT=29.166 min) were separated with good resolution in a single chromatographic run of 50 min. Each drug obeys Beer-Lambert's law in the linearity range of 0.080–0.12 mg/ml for dextromethorphan HBr with a correlation coefficient of 0.998, 0.040–0.060 mg/ml for phenylephrine HCl with a correlation coefficient of 0.996, and 0.010–0.015 mg/ml for triprolidine HCl with a correlation coefficient of 0.998. The recoveries for dextromethorphan HBr and phenylephrine HCl were found to be 99.35% and 99.53%, respectively and for triprolidine HCl was 98.88%. Validation results show satisfactory linearity, precision, accuracy and specificity.



Gradient RP-HPLC, Dextromethorpan hydrobromide, Phenylephrine hydrochloride, Triprolidine hydrochloride, Simultaneous estimation.


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