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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 2
First page : ( 560) Last page : ( 564)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00105.5

Single Nucleotide Polymorphism of Leptin Receptor Gene in Type 2 Diabetes Mellitus: A Cross-sectional Study

Bansal Radhika1, Mayur Sriprajna2, Adiga Usha3,*, Rao Sudheendrs4

12nd MBBS Student, KS Hegde Medical Academy, Nitte-Deemed to be University

2Research Scholar, KS Hegde Medical Academy, Nitte-Deemed to be University

3Professor, Department of Biochemistry, KS Hegde Medical Academy, Nitte-Deemed to be University

4Professor, Department of Medicine, KS Hegde Medical Academy, Nitte-Deemed to be University

*Corresponding Author E-mail: ushachidu@yahoo.com

Online published on 9 April, 2020.

Abstract

Introduction

Type 2 diabetes mellitus (T2DM) is a chronic disease associated with abnormal glucose metabolism due to insulin resistance. Leptin has been shown to be involved in pathways influencing the risk of cardiovascular disease and diabetes. The aim of the study was to evaluate pattern of leptin receptor gene polymorphism Gln223Arg in T2DM and to find its association with serum levels of leptin and also to compare leptin levels in diabetics and non-diabetics.

Methodology

The cross-sectional study was conducted in Molecular Genetics division of KS Hegde Medical Academy, Mangalore. Genotyping of leptin receptor was done for Gln223Arg alleles by PCR-RFLP in 39 type 2 diabetics. Serum leptin was assayed using ELISA in 39 cases and 45 non-diabetic controls. Statistical analysis was carried out with Graph pad Instat version 3.

Results

Hardy-Weinberg Equilibrium (HWE) for the LEPR gene variants showed that alleles were in equilibrium. Leptin levels were insignificantly low in diabetics compared to controls. Women in control groups showed significantly higher leptin levels (p<0.05) compared to men. There was no significant association between leptin levels among different genotypic variants.

Conclusion

We could conclude that type 2 diabetes mellitus is not associated with LepR gene polymorphism as well as there is independent relationship between LepR gene polymorphism and leptin levels

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Keywords

Nucleotide, Polymorphism, Diabetes Mellitus, Leptin, Receptor, Gene.

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