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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2020, Volume : 13, Issue : 12
First page : ( 6140) Last page : ( 6146)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.01071.9

Primary cell culture of zebrafish (Danio rerio) as a material for developing H5N1 avian influenza vaccines

Kusala Muhammad Khaliim Jati12, Anson Arif Nur Muhammad12, Nidom Reviany V.23, Indrasari Setyarina23, Astutik Anis F.2, Normalina Irine2, Alamudi Mohammad Y.2, Rukmana Siti2, Rachmawati Kadek24, Santoso Kuncoro P.24, Nidom Chairul A.24*

1Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.

2Professor Nidom Foundation, Surabaya, 60115, Indonesia.

3PT. Riset AIRC Indonesia, Surabaya, 60115, Indonesia.

4Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.

*Corresponding Author E-mail: nidomca@fkh.unair.ac.id

Online published on 15 February, 2021.

Abstract

In recent decades, zebrafish (Danio rerio) has been widely used as a model of biological experiments not only in the immunology and oncology aspects but also experimental animals for infectious diseases in human and fishes. The virus used in this study was the H5N1 avian influenza reverse genetic virus obtained from the Professor Nidom Foundation, Surabaya, Indonesia. We revealed that TCID50 was 3.1623*107/mL, then the virus was developed by inoculating into the primary cell culture and we performed HA test. Samples that showed positive results on the HA test then performed RNA extraction and confirmed by one-step RT-PCR. It can be seen from the presence of cytopathogenic effects (CPE) in primary cell cultures and also can be seen from HA titers in viral harvest samples from the primary cells of zebrafish (Danio rerio) that shows the same result of HA titers is 28. In conclusion, the present study demonstrated that there is an alternative development of new primary cell sources which leads to halal aspects of the production of avian influenza H5N1 vaccines.

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Keywords

Danio rerio, Primary Cells, H5N1.

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