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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2020, Volume : 13, Issue : 12
First page : ( 5958) Last page : ( 5962)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.01040.9

Isolation and biochemical characterization of an acidophilic, detergent-stable amylase-producing strain of Providencia rettgeri from the soil of patnitop region, J&K

Anwar Afreen1, Dhanjal Daljeet Singh1, Singh Reena1, Chopra Chirag1*

1School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.

*Corresponding Author E-mail: chirag.18298@lpu.co.in

Online published on 15 February, 2021.

Abstract

Amylases are almost ubiquitous and their vital role in biotechnological industries and process such as starch saccharification, detergent, food, and textile industry have prompted the isolation of microbes from different niches. Thus, our current study focusses on the isolation of amylase producing bacteria from Patnitop region. After isolation, the bacterial strain was characterised, during which the highest activity of the enzyme was found to be at 40oC and pH 2.0. The metal ion Mg2+ induced amylase activity whereas detergents like SDS, CTAB could not inhibit the enzyme. The bacterial strain showed significant stability under acidic conditions. On performing 16S rRNA sequencing, the amylase producing bacteria were identified to be Providencia rettgeri.

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Keywords

Amylase, Characterization, Jammu and Kashmir, Metagenomics, Providencia rettgeri.

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