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Year : 2020, Volume : 13, Issue : 12
First page : ( 5893) Last page : ( 5897)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.01028.8

Phytochemical screening and gc-ms analysis of Epiphyllum oxypetalum flower extracts

Jayashree P.1, Shalini M.1, Meenambiga S. S.1*, Suganya V.1

1Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Chennai, Tamil NaduIndia.

*Corresponding Author E-mail: meenambiga.se@velsuniv.ac.in

Online published on 15 February, 2021.


The present study was carried out in Epiphyllum oxypetalum flower to analyse the bioactive compounds present in it. Epiphyllum oxypetalum is a unique plant which blooms only in the night month of June to September. The bioactive compounds obtained from medicinal plant have been treated for various ailments caused by microorganism and it has been analysed with various part of the plant but not in the flower of this plant. Extraction was carried out by using flower sample using maceration method with solvents such as chloroform, ethanol, hexane and aqueous. The crude extracted sample was tested for phytochemical activity, and it showed the presence of compounds such as alkaloids, saponins, proteins and amino acids, terpenoids, steroids, flavonoids, and tannins. Thin layer Chromatography and Column chromatography was performed with the formation of 4 bands in the ethanol extract. The purified sample has been further analysed using Gas chromatography-Mass spectrometry [GC-MS] to check the phytocompounds present in the sample. The first compound identified with less retention time (2.86min) was Hexadecanoic acid, ethyl ester and the last compound which took long retention time (30.81min) was Pterin-6-carboxylic acid. Thus, these plant compounds could be isolated in future to explore their diverse biological activities.



Epiphyllum oxypetalum, Phytochemical activity, Bioactive compound, Gas Chromatography-Mass Spectrometry, Thin Layer Chromatography.


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