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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2020, Volume : 13, Issue : 12
First page : ( 5866) Last page : ( 5870)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.01022.7

The use of DNA sequencing to confirm the first report of rev.1 strain isolated from human brucellosis in Iraq

Jafar Nihad Abdulhussain1, Noomi Bashar Sadeq2, Ali Al-Assie Akeel Hussain3, Sadiq Shadman Tariq4*

1Department of Microbiology, College of Veterinary Medicine, Tikrit University.

2Department of Microbiology, College of Veterinary Medicine, Tikrit University.

3Department of Biology, College of Science, Tikrit University, Iraq.

4Department of Industrial and Fundamental Microbiology, Institute of Science, Ege University, 35040Izmir, Turkey.

*Corresponding Author E-mail: shadmantsm@tu.edu.iq

Online published on 15 February, 2021.

Abstract

Live attenuated Brucella melitensis Rev. 1 strain is currently used in Iraq against caprine and ovine brucellosis. Vaccination with Rev. 1 may lead to the adverse effects of strain persistence in the vaccinated animals and its occasionally spread horizontally. Moreover, in some countries it was shown that the vaccine strain caused human infection, demonstrating the zoonotic hazards of its virulence. Thirty Sheep milk samples and 30 Goat milk samples were taken from animals given a positive result with rose Bengal test. Also 12 Vaginal swabs from aborted ewes and 20 Vaginal swabs from aborted goats were taken in period didn't exceed 10 days after the abortion. At the same time, 50 blood samples taken from patients with Malta fever. The samples cultured on trypton soya broth and then sub-cultured on Brucella Basel agar. Biochemical test, PCR-RELP, and DNA sequencing were used to distinguish Vaccine Rev. 1 strain from field strains. Out of 92 samples from goats and sheep, Brucella isolated from 43 (46.7%) case. The results revealed that vaginal discharges are better than milk samples for isolation of the Brucella. In human, the bacteria isolated from 11 (22%) of Malta fever patients. PCR-RELP and DNA sequencing revealed that Rev.1 strain isolated from 8 (21%) animals and 1 (11.1%) of human patients.

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Keywords

Brucellosis, Brucella melitensis, 16s gene, PCR-RELP, Rev.1, DNA sequencing.

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