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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2020, Volume : 13, Issue : 12
First page : ( 5763) Last page : ( 5767)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.01004.5

Phytochemicals, antimicrobial and antioxidant potential of methanolic extract of Berberis aristata roots

Thakur Monika1, Sharma Kunjan1, Mehta Sonia1, Rai Swati1, Sharma Isha1, Tripathi Astha1*

1Assistant Professor, Faculty of Applied Sciences and Biotechnology, Shoolini University, Bajhol, Solan, Himachal PradeshIndia

*Corresponding Author E-mail: asthatripathi4u@gmail.com

Online published on 15 February, 2021.

Abstract

The present study focuses on exploring the presence of phytochemicals, antioxidants and antimicrobial potential of methanolic extract of root of Berberis aristata. The preliminary qualitative analysis of phytochemicals, free radical scavenging activity, antimicrobial potential and FTIR spectrophotometry were investigated from the root extracts of Berberis aristrata. Methanolic extract of root showed the presence of phytochemicals like alkaloids, carbohydrates, flavonoids, phenolic compounds and saponins. Further antioxidant assay like DPPH (Diphenyl -2-Picrylhydrazyl) assay was done. DPPH free radical scavenging activity was expressed in % inhibition. In present study, the % inhibition of methanolic extract was 52% and IC50 value was 26^g/ml. Antimicrobial screening showed good positive results with tested pathogens Staphylococcus aureus, Pseudomonas areginousa, Klebsiella pneumoniae and Candida albicans. The FTIR (Fourier Transform Infrared Spectrophotometer) spectroscopic studies revealed different characteristic peak values which confirmed that there is presence of various phytocompounds such as lipids, proteins in the methanolic root extracts. The FTIR method was performed on a spectrophotometer system, which was used to detect the representative peak values along with their functional groups. The results of the present study generated the FTIR spectrum profile for the medicinally important plant Berberis aristata. This preliminary study shows that root part of Berberis aristata is a promising herb with many phytochemicals, antioxidants and antimicrobial potential.

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Keywords

Berberis aristata, Phytochemical, Antimicrobial, Antioxidants, FTIR, DPPH.

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