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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2020, Volume : 13, Issue : 12
First page : ( 5699) Last page : ( 5704)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00992.0

HPTLC fingerprinting of quercetin and comparative assessment of anthelmintic potency against Eudrilus eugeniae of Schleichera oleosa (Lour) Oken and Tagetes eretca linn

Goswami Shambaditya1*, Singh Ravindra Pal2, Gilhotra Ritu M.3

1Research Scholar, Suresh Gyan Vihar University, Jaipur, RajasthanIndia

2NIMS Institute of Pharmacy, NIMS University, Rajasthan, JaipurIndia.

3School of Pharmacy, Suresh Gyan Vihar University, Jaipur, RajasthanIndia

*Corresponding Author E-mail: shambampharma@gmail.com

Online published on 15 February, 2021.

Abstract

Helminthiasis is a great burden in current era as it causes enormous health threats. Schleichera oleosa (Lour.) Oken and Tagetes erecata Linn are the medicinal plants which are traditionally used for the treatment of helminthiasis. They are commonly known as ‘Kusum’ and ‘Genda’ respectively. The present research focused on the evaluations of anthelmintic potency of both the plant extracts against an earthworm, Eudrilus eugeniae. The successive extraction method with different solvents (petroleum ether, chloroform, acetone, ethanol and water) is used to prepare the extracts. The extracts were evaluated for the anthelmintic potency at 25, 50 and 100 mg/ml concentrations. Albendazole was used as standard drug. All the solutions were prepared in 3% tween 80 in normal saline solution and alone this solution was considered as control. All the earthworms (n=6) were taken in separate petri-dishes containing extracts, standard and control solution. Paralysis and death time were noted as result in minutes. Standard HPTLC technique (using CAMAG LINOMAT 5) was used to isolate and identify of quercetin from the ethanolic extracts of both the plants. High extractive yields were observed for ethanolic extracts in both the plants. Significant outcome was observed for the evaluation of anthelmintic activity for all the extracts. S. oleosa ethanolic extract showed maximum potency than T. erecta extracts at all concentrations. Dose responsive results were noted. At 100 mg/ml concentration ethanolic extracts of both plants were significant against E. eugeniae. Quercetin was identified in both ethanolic extracts by HPTLC analysis. Structural elucidation of the quercetin and identification of other constituents should be the future prospect.

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Keywords

Helminthiasis, Schleichera oleosa (Lour.) Oken, Tagetes erecata Linn, Eudrilus eugeniae, Anthelmintic potency, HPTLC, Quercetin.

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