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RESEARCH JOURNAL OF PHARMACY AND TECHNOLOGY
Year : 2020, Volume : 13, Issue : 12
First page : ( 5680) Last page : ( 5684)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00989.0

Rutin imparts attenuation of methyl parathion-induced base-pair substitution

Nair Amritha1, Naik Prashantha1*

1Department of PG Studies and Research in Biosciences, Mangalore University, Mangalagangothri-574199, Mangalore, Karnataka, India.

*Corresponding Author E-mail: pn.bioscience.research@gmail.com

Online published on 15 February, 2021.

Abstract

Pesticides plays an important role in the modern agricultural system. Methyl parathion (MP), an organophosphorus insecticide, reported to impose various adverse effects on non-target organisms, including mutagenicity. In the present study, the possible antimutagenic activity of rutin, a flavonoid with potential antioxidant activity, was assessed against MP-induced mutagenicity by employing the Ames test. Salmonella typhimurium -TA 100 was used as the test strain representing the base-pair substitution. A concentration-response toxicity was performed by selecting 0.1, 0.5 and 1.0mg/ml of MP. The protective effect of rutin was assessed at 5, 10 and 20mg/ml against 0.5mg/ml of MP-induced mutagenicity with and without metabolic activation. The results indicated that MP induced concentration-dependent mutagenicity up to 0.5mg/mL (p<0.001). However, growth inhibition was observed in the highest concentration of MP, both in the presence and absence of S9 fraction, indicating the lethality. Rutin induced a concentration-dependent decrease in the frequency of revertant colonies at a significant level up to 1.0mg/ml (p<0.05 and p<0.01) compared with that of MP-treated strains in the presence of S9 fraction. Thus, rutin has the potency to minimize the base-pair substitution induced by MP after metabolic activation, with a threshold limit.

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Keywords

Methyl parathion, Ames test, Antimutagenicity, Rutin, Base-pair substitution.

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