Effect of catechin isolate from gmb4 clone green tea on oxidative stress and apoptosis in experimental cataract Cahyana Nugraha Wahyu12*, Widjajanto Edi3, Kalsum Umi4, Prayitnaningsih Seskoati5 1Doctoral Program of Medical Science, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia 2Department of Ophthalmology, Faculty of Medicine, Jember University, Jember, East Java, Indonesia 3Department of Clinical Pathology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia 4Doctoral Program of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia 5Department of Ophthalmology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia *Corresponding Author E-mail: nugraha.nwc@gmail.com
Online published on 28 October, 2020. Abstract The purpose of this research is to investigate the effect of catechin Isolate from GMB4 Clone Green Tea on apoptotic cell death and oxidative stress in the lens of rats with cataract. Cataract Isolate from GMB4 Clone Green Tea was induced by intraperitoneal injection of 19μmol/kg sodium selenite to ten day-old Wistar rats. The neonatal rats were randomly divided into five groups (n=5 in each group): a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200mg/kg catechin Isolate from GMB4 Clone Green Tea. We performed slit-lamp bio microscopic analysis, level of GSH and GR, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and immunohistochemistry for caspase-3. Both eyes of all rats in Group 1 did not exhibit cataract formation. In Group 2, one out of five (20%) developed grade 3 cataracts and the remaining four out of five (80%) developed grade 4 cataracts. The difference in exhibited cataract in the lens of all rats between Group 2 and any eyes of groups 3 or 4 and 5 were significant (P = 0.022, 0,001, 0,001). The grade of cataract formation was decrease in group 3, 4 and 5. The mean GSH and Caspase-3 levels lenses of group II rats were significantly (P < 0.01) lower than the levels in Group I, Group III, Group IV and Group V. Apoptotic cell death and oxidative stress in the lens that increased following cataract formation in rats was suppressed by cathechin Isolate From GMB4 Clone Green Tea. Top Keywords Apoptosis, Oxidative Stress, Cataract, Catechin Isolate, Sodium selenite. Top |