Characterization of the global metabolic profile of Canagliflozin in Rat plasma, urine and feces based on HPLCUV-MS Analysis Akasha Rawa1,*, Allaf Abdul Wahab2, Al-Mardini Mohammad Amer1 1Department of Pharmaceutical, Chemistry and Quality Control, Faculty of Pharmacy, Damascus University, Damascus, Syria 2Department of Pharmaceutical Chemistry and Quality Control, Faculty of pharmacy, Arab International University, Ghabaghib, Daraa, Syria *Corresponding Author E-mail: Rawaokas@gmail.com
Online published on 24 February, 2020. Abstract Canagliflozin is a newly approved drug for type II diabetes mellitus, in the present work, a strategy based on high performance liquid chromatography combined with mass spectrometry (HPLC-UV/MS) method was proposed to systematically characterize the in vivo metabolites of canagliflozin in Rats. After oral administration of Canagliflozin to rats in a single dose of 3mg/kg, the rat plasma, urine, feces samples were collected and used in order to to determine metabolites. As a result of that and besides the parent drug, a total of 12 metabolites of canagliflozin were detected and identified.it was indicated that the metabolic pathways of canagliflozin in rats included: oxidation (as a main rout), glucuronation, and hydroxylation The result could provide valuable information regarding the metabolism of canagliflozin in rats, which contribute to better understanding of its action mechanism. Top Keywords Canagliflozin, metabolites, diabetes, HPLC-UV/MS, sodium glucose co-transporter 2 inhibitor. Top |