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Cell Viability Studies and Anti-cancerous activity Evaluation of Pomegranate (Punica granatum L) extract Pinnamaneni Rajasekhar* Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Green-fields, Vaddeswaram, Guntur, Andhra Pradesh-522 502, India *Corresponding Author E-mail: pinnamaneniraj@yahoo.com
Online published on 24 February, 2020. Abstract Pomegranate, Punica granatum L belonging to the family Lythraceae is a widely used plant having medicinal properties. Pomegranate gained considerable recognition as a functional food in the modern era. In light of the importance of pomegranate extract as an anti-cancerous agent, an attempt was made to analyze its anti-cancerous activity by studying the toxicity studies on the cancerous cell lines by treating with pomegranate peel extract and studying the viability of cancerous cell lines. Dried pomegranate peel extract powder was obtained by rotary evaporation and freeze drying. The percentage viability of HCT 15 and T47D cancer cells carried out by using Trypan blue staining were 73.46%, 78.35% respectively which are most suitable to perform cytotoxicity studies. With the increase in the concentration of the pomegranate peel extract (20 to 120 μl/ml acetone, methanol and ethyl acetate pomegranate peel extracts); the percent viability of cells tends to decrease. Top Keywords Pomegranate extract, Cell viability, Trypan blue staining, MTT assay. Top | |
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