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Research Journal of Pharmacy and Technology
Year : 2020, Volume : 13, Issue : 1
First page : ( 275) Last page : ( 281)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2020.00056.6

A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin

Rantam Fedik Abdul1,2,*, Nugraha Alexander Patera2,3,4, Ferdiansyah Ferdiansyah5, Purwati Purwati2,6, Bumi Candra2, Susilowati Helen2, Hendrianto Eryk2, Utomo Dwikora Novembri5, Suroto Heri5, Sumartono Christijogo7, Setiawati Rosy8, Prakoeswa Cita Rosita9, Indramaya Diah Mira9

1Virology and Immunology Laboratory Microbiology Department., Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

2Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, 60115, Indonesia

3Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

4Orthodontic Department Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

5Orthopedic and Traumatology Department, RSUD Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

6Health Department Faculty of Vocation, Universitas Airlangga, Surabaya, 60132, Indonesia

7Anasthesiology and Reanimation Department, RSUD. Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

8Radiology Department, RSUD Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

9Dermatology and Venerology Department, RSUD Dr. Soetomo Hospital, Faculty of Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia

*Corresponding Author E-mail: fedik-a-r@fkh.unair.ac.id

Online published on 24 February, 2020.

Abstract

Human Adipose Derived Mesenchymal Stem Cells (HADMSCs) and Human Hair Follicle Derived Mesenchymal Stem Cells (HHFDMSCs) have attracted great interest because of their multilineage differentiation potential, selfrenewal properties, and their possible use of cell and gene therapies. This present study to investigate the neurogenic differentiation ability of hADMSCs and hHFDMSCs induced by Epidermal Growth Factors (EGF), Fibroblast Growth Factor (FGF), Platelet Derived Growth Factor (PDGF) and Forskolin. This study was true experimental with longitudinal study design. The sample size determined with minimal sample size formula and it was randomly chosen. These studies employed an in vitro design for the expansion and proliferation of Mesenchymal Stem Cells (MSCs) and examined the heterogeneity of these cells using the markers CD105, CD90, OCT4, and SOX2. MSCs from adipose tissue and hair follicles were induced with EGF, FGF, PDGF and Forskolin to differentiate and generate neurons. The capacity of MSCs to generate neurons were verified using glial fibrillary acidic protein, nestin, and β-tubulin III. The expression of neural markers and morphological changes in Mesenchymal stem cells from hADMSCs and hHFDMSCs were confirmed. hADMSCs and hHFDMSCs share a similar capacity to differentiate and generate neurons, which is beneficial for the development of neuronal restoration for future therapies for patients suffering from neurological diseases.

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Keywords

Human Adipose Mesenchymal Stem Cells, Human Hair Follicle Mesenchymal Stem Cells, Proliferation and differentiation, Growth Factor, Neurons.

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