Novel Stability indicating RP-UPLC Method for simultaneous Determination of Ivabradine and Metoprolol drug materials in bulk and their Pharmaceutical Dosage Forms Gandi Suresh1,*, Manikandan A.1, Rao S. Venkat2 1Bharath Institute of Higher Education and Research, Selaiyur, Chennai-600073, India 2SMS Pharmaceuticals Ltd, R&D Centre (A DSIR approved), Gagillapur, Telangana-500047, India, svr.org@gmail.com *Corresponding Author E-mail: suresh84gandi@yahoo.co.in
Online published on 24 February, 2020. Abstract A simple, Accurate, precise method was developed for the simultaneous determination of the Ivabradine and Metoprolol in Pharmaceutical dosage form. Chromatogram was run through SB C8 100 x 3.0mm, 1.8μm. Mobile phase containing Buffer 0.01N Potassium dihydrogen ortho phosphate: Acetonitrile chosen in the ratio 50: 50v/v was pumped through column at a flow rate of 0.3 ml/min. Temperature and Wave length was optimized at 30°C and 260 nm independently. Percentage of recovery was obtained as 100.03% and 100.03% for Ivabradine and Metoprolol individually. LOD, LOQ values obtained from regression equations of Ivabradine and Metoprolol were 0.03, 0.08 and 0.12, 0.35 respectively. Top Keywords New method development, Determination, Ivabradine, Metoprolol, RP-UPLC. Top |