Carbohydrates determination in the Snowdrops (Galanthus L.) herbal pharmaceutical substances by TLC and UV-Spectrophotometry Bokov D.O.1,2, Kulaeva I.R.3, Potanina O.G.4, Sergunova E.V.1, Bondar A.A.1, Evgrafov A.A.1, Antsyshkina A.M.1, Krasnyuk I.I.1 1Institute of Pharmacy, Sechenov First Moscow State Medical University, 8 Trubetskaya St., bldg. 2, Moscow, 119991, Russian Federation 2Laboratory of Food Chemistry, Federal Research Center of Nutrition, Biotechnology and Food Safety, 2/14 Ustyinsky pr., Moscow, 109240, Russian Federation 3Medical Institute, Chechen state University, 32 Sheripova St., Grozny, Chechen Republic, 364061, Russian Federation 4Pharmaceutical chemistry and pharmacognosy chair, Peoples’ Friendship University of Russia (RUDN University), 6, Miklukho-Maklaya Street, Moscow, 117198, Russian Federation Corresponding Author E-mail: fmmsu@mail.ru
Online published on 24 February, 2020. Abstract Plants of Galanthus L. genus (snowdrops) used in Russian medicine for obtaining neurological and cardiovascular remedies, contain different types of carbohydrates (polysaccharides, monosaccharides) as additional biological active substances group. In present research the carbohydrate composition and content were investigated in the snowdrops herbal pharmaceutical substances (Galanthus woronowii Losinsk, Galanthus nivalis L.). Optimal conditions for TLC analysis, the extraction of crude herbal drugs, were determined; spectrophotometric procedures for quantifying the total carbohydrate content in terms of monosaccharides (fructose, glucose) were successfully developed. The results of the investigation can be used in quality control and standardization of crude herbal drugs containing carbohydrates, and for inclusion in pharmacopoeial monographs. Top Keywords carbohydrates, Galanthus woronowii Losinsk, Galanthus nivalis L, herbal pharmaceutical substances, glucose, fructose. Top |