Pharmacognostical Studies and Isolation of an alkaloid from Barleria cristata Linn. roots Banu Shanaz1,*, Jenita Josephine Leno2, Manjula D.2, Premakumari K. B.3 1Department of Pharmacognosy, Dayananda Sagar University, Bangalore-560078, India 2Department of Pharmaceutics, Dayananda Sagar University, Bangalore, India 3Department of Pharmaceutical Analysis, Dayananda Sagar University, Bangalore, India *Corresponding Author E-mail: shanaz2906@gmail.com
Online published on 24 February, 2020. Abstract The present investigation was carried out to study the histological characters of the roots of the plant Barleria cristata Linn. Further the phytochemical studies on the methanolic extract of roots revealed the presence of alkaloids. Column chromatography of methanolic extract of Barleria cristata Linn. roots (MEBC) was carried out by using silica gel (60–120#) column chromatography. It was eluted by different solvents in their increasing order of polarity. Fraction No.11 was purified by fractional crystallization to yield 55mg of brown coloured amorphous powder of Compound-I which was characterized and confirmed as Ibogamine by TLC, melting point, Infrared Spectroscopy, 1H NMR, 13C NMR and Mass spectrometry. Ibogamine, an alkaloid was isolated from the roots of Barleria cristata Linn. roots. Top Keywords Barleria cristata Linn, column chromatography, ibogamine, alkaloid, standardization. Top |