Ultrastructural Characteristics of HPV in Women's Vaginal Cells Park Mi-Soon1, Chang Byung-Soo2, 1Department of Pathology, Korea Clinical Laboratory, Seoul, 05396, Korea 2Department of Cosmetology, Hanseo University, Seosan, 31962, Country Corresponding Author E-mail: Kimpark42000@naver.com, bschang@hanseo.ac.kr
Online published on 24 December, 2019. Abstract Background/Objectives This study examines the microstructural characteristics of HPV-infected koilocytes microscope taken from the smear test of women's vaginal cells and the shape of HPV particles under a light. Methods/Statistical Analysis Koilocytes infected with human papilloma virus were stained with Papanicolaou's method and examined by light microscopy. Ultrastructural characteristics of human papilloma virus inside the koilocyte were identified using transmission electron microscopy. Findings The cytoplasm of a koilocyte infected with activated virus was filled with keratin filaments and some vacuoles and ribosomes. Activated HPV particles were clearly observed inside the nucleus. These virus particles were identified as spherical, with a diameter of 50 nm, and were mainly around nuclear membrane and nucleus. Improvements/Applications This study identified that, as HPV invades and proliferates inside the nucleus of epithelial cells, the dysplasia of epithelial cells into koilocytes by forming a nucleophilic zone. Top Keywords Human papilloma virus, Koilocyte, Papanicolaou stain, TEM, Vagina. Top |