Cytotoxic and Xanthine Oxidase Inhibitory Activities of Chemical Constituents of Alphonsea cylindrica King Bakri Yuhanis Mhd1, Aldulaimi Ahmed Kareem Obaid1,*, Azziz Saripah Salbiah Syed Abdul1,*, Nafiah Mohd Azlan1, Aowda Saadon Abdulla2, Hazim Muhammad1, Awang Khalijah3, Hashim Yumi Zuhanis Has-Yun4, Abbas Phirdaous5 1Department of Chemistry, Faculty of Science and Mathematics, Sultan Idris Education University, 35900, Tanjong Malim, Perak, Malaysia 2Department of Chemistry, Faculty of Science, University Of Babylon, Babylon, Iraq 3Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia 4International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, P.O Box 10, 50728, Kuala Lumpur, Malaysia 5Department of Biotechnology Engineering, Kul. of Engineering, International Islamic University Malaysia, P.O Box 10, 50728, Kuala Lumpur, Malaysia *Corresponding Author E-mail: ahmedaldulaimi1@gmail.com, saripah@fsmt.upsi.edu.my
Online published on 24 December, 2019. Abstract This study aimed to investigate the chemical constituents from Alphonsea cylindrica as well as their cytotoxic and xanthine oxidase inhibitory activities. The chemical compounds were isolated and purified by various chromatographic techniques and their structures were elucidated via modern spectroscopic techniques including NMR, MS, IR, UV and comparison with literature. A study on the barks of A. cylindrica has found eleven known compounds were identified as stigmasterol (1), isoursuline (2), cyathocaline (3), kinabaline (4), muniranine (5), isooncodine (6), iraqiine (7), O-methylmoschatoline (8), kareemine (9), atherospermidine (10) and N-methylouregidione (11). The cytotoxic effect of hexane, DCM and methanol of bark of A. cylindrica as well as the isolated compounds; 1, 2, 3, 4, 5, 7, 8, 9 and 10 were estimated on MCF-7 human breast cancer cells and isoursuline possessed the most potent inhibitory activity with IC50 value of 33 μg/ml. Eight compounds 1, 3, 4, 5, 6, 8, 10 and 11 displayed moderate inhibitory activity effect with IC50 < 100 μM against xanthine oxidase. As a conclusion, eleven compounds have been isolated from the bark of A. cylindrica King. Some of the compounds also showed potential against cytotoxicity and xanthine oxidase inhibitory activities. The findings of this study can enhance the understanding of the chemotaxonomy aspect of the A. cylindrica as well as its potential in traditional and modern medicine. Top Keywords Alphonsea cylindrica, Alkaloid, Cytotoxic, MCF-7, Xanthine Oxidase. Top |