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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 8
First page : ( 3687) Last page : ( 3689)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00629.2

A Study on Rickettsial Infections and its Isolation by Serological and Molecular Methods

Shahid S. Mohammed1, Dr. Sirwar Siddesh B.2,*, Dr. Vijayaraghavan R.3

1Assistant Professor, Dept. of Microbiology, Khaja Bandanawaz Institute of Medical Sciences, Gulbarga-585104 and Ph.D Scholar, Saveetha University, Chennai-602105

2Professor and HOD, Dept. of Microbiology, Khaja Bandanawaz Institute of Medical Sciences, Gulbarga-585104, Karnataka

3Director of Research, Saveetha University, Thandalam, Chennai, 602105. India

*Corresponding Author E-mail: drsidd1110@gmail.com

Online published on 24 December, 2019.


Background and objectives

Prevalence of re-emerging of rickettsial infections are increased the major clinical manifestations present times in India.


To study the incidence, prevalence and isolation by serological and molecular methods. Methodology All the suspected cases clinically were subjected for Weil-Felix test and further confirmation by isolation by molecular method.


The study depicts pattern in clinically signs and symptoms with fever, chills and rash in majority cases, along with incidence predominant in above 30 years in male and female group. Out of 262 cases 116 positive by Weil-Felix test and among them 69 showed positive for gltA gene by PCR.


Detection of gltA gene is ideal target for the diagnosing of rickettsial infections in early phase of the infection because of its high sensitivity and specificity.



Rickettsia, rash, fever with chills, history of insect bite Weil-Felix test, citrate synthase gene (gltAgene), PCR test.


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