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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 8
First page : ( 3629) Last page : ( 3636)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00619.X

Preliminary Phytochemical Analysis and Isolation and Identification of active principle components of petroleum ether extract from leaves of Lawsoniainermis L.

Arirudran B.1, Krishnan V.2, Rao Us Mahadeva3,*

1Assistant Professor, P.G. Department of Biochemistry, SRM Arts and Science College, Kattankulathur, Kanchipuram District, Tamil Nadu-603203

2Post Graduate Student, P.G. Department of Biochemistry, SRM Arts and Science College, Kattankulathur, Kanchipuram District, Tamil Nadu-603203

3Professor (VK7), School of Basic Medical Sciences, Faculty of Medicine, University of Sultan Zainal Abidin, 20400, Terengganu, Malaysia

*Corresponding Author E-mail: raousm@gmail.com

Online published on 24 December, 2019.



Lawsoniainermis L belongs to the Lythraceae family, generally known as Henna, cultivated for its leaves although stem, bark, roots, flowers and seeds. They were used in traditional medicine to treat a variety of ailments as rheumatoid arthritis, headache, ulcers, diarrhea, leprosy, fever, leucorrhoea, diabetes, cardiac disease. They were used for dye making.


The present study focus on preliminary phytochemical analysis and determination of bioactive components from the leaf of Lawsoniainermis L.


Preliminary phytochemical analysis and identification of active components by GC/MS were done.


The preliminary phytochemical analysis reveals that the aqueous and petroleum ether extracts from leaf of Lawsonia inermis Lconsists of alkaloids, anthraquinones, flavonoids, phenols, reducing sugars, saponins, steroids, tannins, and terpenoids.GC/MS analysis reveals that petroleum ether extracts consists of phthalic anhydride, 4methylformanilide, acetamide, N-(2-methylphenyl)-, 3-hydroxycarbofuran, 2-hydroxy-1,4-naphthalenedione, dodecyl acrylate,3,7,11,15-tetramethyl-2-hexadecen-1-ol, trans-geranylgeraniol, n-hexadecanoic acids, 9,12,15octadecatrienoic acids, (Z,Z,Z)-octadecanoic acids, phytol, acetate, cis-13-eicosenoic acid, cyclohexadecane-1,2diethyl-, 4,8,12,16-tetramethylheptadecan-4-olide, eicosane, tetratriacontane, supraene, 1,6,10,14hexadecatetraen-3-ol, 3,7,11,15-tetramethyl-, (E,E)-, stigmastan-3,5-diene, triacontane, and vitamin E.


This study shows that Lawsoniainermis L. leaves contain bioactive phytochemicals that may serve as a biocompatible and eco-safe agent in formulation of drugs, natural dyes in colouration of textile materials and other purpose.



Bioactive components, Phthalic anhydride, 2-Hydroxy-1, 4-Naphthalenedione.


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