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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 7
First page : ( 3413) Last page : ( 3417)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00577.8

Development and Validation of RP-HPLC Method for Determination of Ritonavir and Lopinavir

Ayeen Fathima Qurratul1,*, Dr. Yasmeen Ruheena2, Badar Humera3

1Assistant Professor, Department of Pharmaceutical Analysis and Quality Assurance, Nizam Institute of Pharmacy, Deshmukhi (V), Pochampally (M), Behind Mount Opera, Yadadri Bhuvanagiri (Dist)-508284, Telangana, India

2Assistant Professor, Department of Pharm. D (Pharmacy Practice), Nizam Institute of Pharmacy, Deshmukhi (V), Pochampally (M), Behind Mount Opera, Yadadri Bhuvanagiri (Dist)-508284, Telangana, India

3Assistant Professor, Department of Pharmaceutical Analysis and Quality Assurance, Nizam Institute of Pharmacy, Deshmukhi (V), Pochampally (M), Behind Mount Opera, Yadadri Bhuvanagiri (Dist)-508284, Telangana, India

*Corresponding Author E-mail: ayeenfathima@gmail.com

Online published on 24 December, 2019.

Abstract

A novel simple, accurate, precise and selective high performance liquid chromatographic (HPLC) method was developed and validated for simultaneous estimation of Ritonavir and Lopinavir in tablet dosage form. An isocratic, reverse phase HPLC method was developed and validated using Hypersil C18 (250 mm×4.6 mm i.d.) 5μm, column and orthophosphoric acid pH3 and methanol (40:60) as mobile phase and detection is carried out at a wavelength of 273nm. The retention time for RITO and LOPI were 3.3±0.1 min and 4.7±0.1min respectively. The method was validated with respect to linearity, precision, accuracy and robustness.

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Keywords

Ritonavir, lopinavir, HPLC, UV, orthophosphoric acid (OPA).

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