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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 7
First page : ( 3389) Last page : ( 3392)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00572.9

Spectrophotometric Determination of Amlodipine Besylate in Pure Form and Pharmaceutical Formulation using Amido Black

Alkhalil Raghad1, Attal Aya1, Sakur Amir Alhaj2,

1Chemistry Department Faculty of Sciences, University of Aleppo, Syria

2Analytical and Food Chemistry Department Faculty of Pharmacy, University of Aleppo, Syria

Corresponding Author E-mail: profsakur@gmail.com, rag.h@hotmail.com, ayaattal15@gmail.com

Online published on 24 December, 2019.

Abstract

Objective

Simple, rabid and sensitive spectrophotometric method have been developed for the determination of Amlodipine Besylate (AMD) both in pure and in pharmaceutical forms.

Methods

spectrophotometric method is based on the formation of chloroform extractable blue colored ion-pair complex between Amlodipine Besylate (AMD) and Amido Black (AB)with absorption maxima at 592 nm.

Results

The effects of analytical parameters on the reported system were investigated. The complexation reaction was extremely rapid at room temperature and the absorption values remain unchanged up to 24h. Various analytical parameters have been evaluated and the results have been validated by statistical. Beer's law was obeyed in the concentration range of 1.0–56 μg.ml−1, the limit of detection was 0.16 μg.ml−1 and the molar absorptivity coefficient was1.92×104 L.moL−1.cm −1.

Conclusion

The proposed method was successfully applied to the analysis of commercial dosage forms (tablets and capsules) containing the drugs with average recovery of 98.66–101.90% with RSD less than 3.84%. The method was chloroform-extractive, rapid, and have high sensitivity comparing with other previous spectrophotometric methods. Interferences of the other ingredients and excipients were not observed.

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Keywords

Spectrophotometry, Amlodipine Besylate, Amido Black, ion-pair complex, extractable complex.

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