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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 7
First page : ( 3350) Last page : ( 3354)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00565.1

Expression of FGF-2 and Fibronectin in Citrus limon Fruit Peel Malang Essential Oil Gel Treated Traumatic Ulcer in Diabetic Wistar Rats (Rattus novergicus)

Ganesha Raziv, Hernawan Iwan, Hendarti Hening Tuti, Radithia Desiana, Hadi Priyo, Ayuningtyas Nurina Febriyanti, Ernawati Diah Savitri*

Department of Oral Medicine, Faculty of Dental Medicine, Airlangga University, Jl. Mayjend Prof Dr. Moestopo 47, Surabaya, East Java, Indonesia

*Corresponding Author E-mail: diah-s-e@fkg.unair.ac.id

Online published on 24 December, 2019.


Diabetes Mellitus is a metabolic disease that delayed traumatic ulcer healing process. Citrus limon contains fumaric acid, d-limonene and citral that can accelerate the healing of traumatic ulcer. The aims of this study were to investigate the effect of 0.78% Citrus limon essential oil topical application can increase Fibroblast Growth Factor-2 (FGF-2) and Fibronectin expression. This study was true experimental analytic study with random sampling methods. The study was conducted on 30 male Wistar rats (Rattus novergicus) induced with DM by injecting 50 mg/kg of Streptozotocin, intraperitoneally, and a traumatic ulcer on their lower lip mucosa. These were divided into six groups; three each for control and treatment groups. Each control and treatment group consisted of five rats. The control groups treated with CMC 5% gel and treatment groups were administered with Citrus limon peel essential oil gel. The expression of FGF-2 and Fibronectin was observed on Days 5, 7 and 9. Furthermore, mice sacrificed and the lower lip labial mucosa tissue of mice has been taken to make the Histopathology Anatomy preparation by means of immunohistochemical examination with monoclonal antibodies anti-FGF2 and anti-Fibronectin. ANOVA analysis was performed to analyze the difference between group (p<0.05). The increased FGF-2 and Fibronectin expression in the treatment group as compared to that of the control group. There were significant differences (p<0.05) of both FGF2 and Fibronectin expression between the two groups. The citrus limon peel essential oil gel increased the FGF-2 and Fibronectin expression during the healing process of traumatic ulcers on the oral mucosa of diabetes afflicted Wistar rats (Rattus novergicus).



Diabetes Mellitus, Citrus limon Essential Oil Gel, Traumatic ulcer, Fibroblast Growth Factor2, Fibronectin, Herbal Medicine.


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