Development and Validation for estimation of Abiraterone acetate in Bulk and Pharmaceutical Dosage Form by UPLC Goud V. Mohan*, Rani B Sandhya, Sharma JVC, Sirisha P. Department of Pharmaceutical Analysis, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist. Telangana *Corresponding Author E-mail: mohanvanga@yahoo.com
Online published on 8 August, 2019. Abstract A simple, Robust, stability indicated method was developed for the estimation of Abiraterone by RP-UPLC technique. Chromatographic conditions used are, stationary phase SB C8 100 × 3.0 mm, 1.8μ. Mobile phase 0.01N NaH2PO4: Acetonitrile in the ratio of 60: 40v/v and flow rate were maintained at 0.3 ml/min, detection wave length was 235 nm, and column temperature was set to 30°C. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150% levels, R2 value was found to be as 0.999. Precision was found to be 0.6 for repeatability and 1.1 for intermediate precision. LOD and LOQ are 0.09μg/ml and 0.27μg/ml respectively. Degradation studies of Abiraterone were done, in all conditions and it is within the acceptable range. Top Keywords UPLC, Abiraterone Acetate, Mobile Phase, Method development. Top |