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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 6
First page : ( 2824) Last page : ( 2827)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00475.X

In vitro Screening of Antioxidant, Anti-Inflammatory activity of Vitex negundo Methanolic leaf extract

Anusha D.1,*, Kancherla Nagaraju2, Chellathai Darling1

1Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, SRIHER, Porur, Chennai-600116

2 Tutor, Dept. of Pharmacology, GSL Medical College, Rajamundhary, A.P.

*Corresponding Author E-mail: drdanusha@gmail.com

Online published on 8 August, 2019.



Vitex negundo L. (verbenaceae), is a large aromatic shrub with various medicinal properties. This current work has been proposed to explore antioxidant property of methanolic extract of Vitex negundo by various in vitro assays.


The in vitro antioxidant action has been determined via DPPH assay, Nitric oxide scavenging activity, and by assessing reducing power. Results Methanolic extract of Vitex negundo showed a very good anti-radical activity in scavenging DPPH radical with maximum % inhibition of 81.87±1.53% at 2000μg/0.1 ml concentration and nitric oxide radical with maximum % inhibition of 75.29±1.33% at 2000μg/ml. Reducing power of Vitexnegundois83.75±0.57% at 2000μg/0.05 ml concentration. Adding on, methanolic extract of Vitex negundo also showed significant anti-inflammatory activity with maximum % inhibition of 79.81 at 500ug.


Taken together the result shows that methanolic extract of Vitex negundo possesses substantial free radical scavenging action and anti-inflammatory properties. Hence more research is warranted for ascertaining the effective compound contributing towards its antioxidant and antiinflammatory activities.



Vitex negundo, Methanolic leaf extract, antioxidant potential, DPPH scavenging activity, NO scavenging activity, total antioxidant activity and reducing power, anti-inflammatory.


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