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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 6
First page : ( 2657) Last page : ( 2663)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00444.X

New Stability Indicating RP-Ultra Fast Liquid Chromatographic Method for the Determination of Perampanel-An Antiepileptic Drug

Annapurna Mukthinuthalapati Mathrusri*, Kaibada Rajani, Srinivas Mandula

Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, Andhra pradesh-530045

*Corresponding Author E-mail: mannapurna.mukthinuthalapati@gitam.edu

Online published on 8 August, 2019.

Abstract

A new RP-UFLC method has been developed and validated for the determination of Perampanel in its capsules using Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector on C8 (2) 100A (Luna) column (250 mm×4.60 mm i.d. 5μm particle size)maintained at room temperature. Mobile phase consisting of a mixture of water, acetonitrile and 0.1% acetic acid (50: 50: 0.1 v/v) was chosen with a flow rate of 1 mL/min (UV detection at 215nm) on isocratic mode. Perampanel obeys Beer-Lambert's law over the concentration range 0.05–120 μg/mL with linear regression equation y=73084x+13800 (r2=0.9999). Forced degradation studies were performed and the method was validated as per ICH guidelines.

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Keywords

Perampanel, RP-UFLC, Stability indicating, Validation, ICH guidelines.

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