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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 5
First page : ( 2131) Last page : ( 2136)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00353.6

Isolation and Identification of mecA gene from MRSA isolated from Local Sheep and Evaluate the Inhibitory effect of Cranberry Leaves extract In-Vitro

Sarhan Sarhan R.1,*, Mohammed Hussein A.2

1Department of Physiology and Pharmacology, College of Veterinary Medicine, Wasit University, Wasit, Iraq

2Department of Microbiology, College of Veterinary Medicine, Wasit University, Wasit, Iraq

*Corresponding Author E-mail: srashid@uowasit.edu.iq

Online published on 8 August, 2019.

Abstract

The current study focused and gave an insight about the distribution percentage of Methicillin-Resistant Staphylococcus aureus or sometimes called (MRSA) in sheep admitted to Veterinary medicine college, Wasit university at the period extended from March 2017 to June 2018 in Wasit province, Iraq and evaluate the inhibitory activity of ethanol extract of cranberry leaves. Consequently, from a total one hundred and fifty specimens, seventy samples were positive for the presence of S. aureus. Moreover, higher rate was detected in the wounds samples 37 (74%) and respiratory samples 20(40%) higher than that found in urine 10 (20%). The results of antibiotics susceptibility test for Staphylococcus aureus were indicated a high and moderate level of resistance to all antibiotics used. Additionally, a polymerase chain reaction was used, as a molecular technique, to detect mecA and results indicated that 54 strains were mecA positive and were labelled as MRSA. Moreover, results indicated that cranberry leaves extract had inhibitory activity against MRSA. In conclusion, the leaves extract of Cranberry has shown powerful antimicrobial activity, and these finding might be encouraging to several applications as antimicrobials or animals feed preservation.

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Keywords

MSRA, PCR, mecA, Bacterial resistance, Cranberry leaves.

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