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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 5
First page : ( 2075) Last page : ( 2078)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00343.3

Simultaneous Estimation of Paracetamol and Zaltoprofen in Pharmaceutical Dosage Form by HPTLC

Premakumari KB1, Mahesh AR1,*, Murugan V1, Ezhilarasan V2

1Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Dayananda Sagar University, Bengaluru

2Lotus Labs, Vasanth Nagar, Bengaluru

*Corresponding Author E-mail: mahesh-sps@dsu.edu.in

Online published on 8 August, 2019.

Abstract

Objective

A method for HPTLC was developed and validated for simultaneous estimation of Paracetamol and Zaltoprofen in a pharmaceutical dosage form.

Methods

The Chromatographic separation was achieved on TLC aluminum plates precoated with silica gel G60 F254 as the stationary phase. The mobile phase consisted of Hexane: Chloroform: Methanol in the ratio of 3.5: 5.5: 1v/v/v.

Results

The method was found to be linear in the concentration range of 0.8–4μg/spot for PCM and 0.2–1 μg/spot for ZLT and the correlation coefficient were found to be 0.996 for PCM and 0.998 for ZLT. The %RSD value obtained for intraday precision were 0.237 and 0.377 and for inter-day precision were 0.259 and 0.766 respectively, the low % RSD indicates that proposed method is precise as per ICH guidelines. The accuracy of the method was determined and the mean recovery of PCM was 99.73–100.15% and ZLT was 99.57–100.18%. LOD and LOQ values were found to be 0.034 μg/spot and 0.105 μg/spot for PCM, 0.006μg/spot and 0.018 μg/spot for ZLT.

Conclusion

The method reported is accurate, precise, specific, robust and linear for the estimation of Paracetamol and Zaltoprofen in Pharmaceutical dosage form.

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Keywords

Paracetamol, Zaltoprofen, HPTLC, Chromatography, HPTLC.

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