Phytochemical Analysis, Standardization and Cytotoxic Activity of Curcuma aureginosa Extract in Human Breast Cancer (MCF-7) Cell Line Isrul Muhammad*, Juliansyah Risky, Saleh Ahmad, Yuliastri Wa Ode, Pusmarani Jastria, Himaniarwati, Maulidina Wa Ode Wahyuni Department of Pharmacy, STIKES Mandala Waluya Kendari, South-East Sulawesi, Indonesia *Corresponding Author E-mail: isrulfar@gmail.com
Online published on 8 August, 2019. Abstract Last several years, researches about the plant as an alternative treatment of breast cancer have been developed. Curcuma aureginosa has been applied empirically as a breast cancer treatment and provide a satisfying result. The aim of this study is to examine cytotoxic activity of C.aureginosa extract to MCF-7 breast cancer cells, phytochemical analysis, and standardization of extract. Examination of breast cancer activity conducted by MTT method, extract was examined at concentration of 2000, 1000, 500, 250, 125, 62.5, 32.25, 15.625, and 7.8125 μg/ml, doxorubicin 1 μg/ml is applied as a positive control. Phytochemical Analysis conducted by GC-MS, and standardization of extract conducted based on Indonesia Pharmacopeia. The results reveal that extract has cytotoxic activity toward MCF-7 breast cancer cell. Concentration of 2000, 1000, 500, 250μg/ml has similar activity with doxorubicin 1 μg/ml. IC50 value of the extract is 76.24 μg/ml with 48 hours incubation. Standardization result determines extract profile and completes the standard of Indonesia Pharmacopeia. Phytochemical analysis result reveals Germacra-1(10), 4-diene-12-oic acid 6 alpha hydroxy gamma lactone (25.12%) and Cycloisolongifolene, 8, 9-dehydro-9-formyl-(21.61%) as a major compound. Cycloisolongifolene, 8, 9-dehydro-9-formyl-, Alpha-Humulene, Curcumene, and Curzerene assumed as cytotoxic toward MCF-7 breast cancer cell. Top Keywords Curcuma aureginosa, Breast cancer, MCF-7, MTT, GC-MS, Standardization. Top |