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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 4
First page : ( 1963) Last page : ( 1966)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00328.7

Screening and Isolation of Fibrinolytic Enzymes from Bacteria using Agrowaste for Thrombolytic Treatment

Priya S. Lakshmi, Prema K. Krishna*

Department of Microbiology, Ethiraj College for Women, Chennai

*Corresponding Author E-mail: kkrishnaprema@gmail.com

Online published on 8 August, 2019.

Abstract

Bacterial fibrinolytic enzymes find great applications to treat and prevent cardiovascular diseases (CVD). The novel food-grade microorganisms are useful for thrombolytic therapy. Agro-residues were used for the production of fibrinolytic enzyme in solid-state fermentation. In this study, four different food samples were used and two Bacillus sp were isolated. The bacterial isolates were cultured and screened for its fibrinolytic activity. Under SSF, the production of fibrinolytic enzyme was enhanced by using cow dung substrate. The enzyme was then further purified by ammonium sulphate precipitation and dialysis method. After the successive purification steps, the molecular weight was estimated. The efficiency of the fibrinolytic enzyme produced was determined by its clot lytic activity on fibrin clot with the known concentration of the standard ‘Streptokinase’.

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Keywords

Corn, Pearl millet (Kambu), Finger millet (Ragi), Wheat, Bacillus subtilis, Solid-substrate fermentation, Cow dung, Fibrinolytic enzyme, SDS-PAGE, Thrombolytic treatment.

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