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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 4
First page : ( 1521) Last page : ( 1528)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00252.X

Vegetable Waste as an Alternate Plant Tissue Culture Media for Laboratory and Industry

Subbaiya R.1,*, Aakash B.1, Shanmugaraja A.2, Devika R.1, Chozhavendhan S.1, Vinoth S.1, Devi G. Karthiga1, Selvam M. Masilamani3, Chozhavendhan S.4

1Associate Professor, Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University) Paiyanoor, Chennai

2Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal-637215, Tamil Nadu, India

3Department of Biotechnology, Satyabhama Institute of Science and Technology, Jeppiaar Nagar, Chennai-600 119

4Associate Professor, Department of Biotechnology, Vivekanandha College of Engineering For Women, Elayampalayam, Tiruchengode

*Corresponding Author E-mail: ramsubbubio@gmail.com

Online published on 8 August, 2019.

Abstract

The clean production concept shows a good utilization potential for solid vegetable waste. It could achieve a reduction of investment and raw materials and can contribute to waste minimised plant production. Thus rendering cheap and environmentally safe alternative to commercial media. At present study was aimed at finding the potential of vegetable waste as a culture media. Composition of the media and the efficiency of the media to support plant growth were tested. Vegetable waste of Cabbage, Beetroot and Onion were collected and dried in the sun light for 2 weeks. Dried vegetable waste was grinded into fine powder. These vegetable waste media (alternative plant tissue culture media) was prepared and coconut water was added for natural hormone (cytokinin). The alternative media pH was 5.6 to 5.7 before sterilization. The vegetable waste powder was analysed for Macronutrients which include potassium (268 mg/kg), sodium (520 mg/kg), calcium (1591 mg/kg), while the micronutrients were analysed copper (5.421 mg/kg), iron (1101 mg/kg) and zinc. The growth was comparable to that obtained on routine commercial media.

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Keywords

Vegetable waste, BCO, plant tissue culture media, cost effective.

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