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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 4
First page : ( 1491) Last page : ( 1494)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00246.4

Multiple Shoot Regeneration in Seed-derived Immature Leaflet Explants of Red Dragon Fruit (Hylocereus costaricensis)

Kasim D Pedda1, Kishore N Sai2, Suneetha P3, Rao K Bramareswara4, Kumar M Naresh1, Krishna M.S.R.1,*

1Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur

2Department of Biotechnology, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh

3Prof. Jaya Shankar Telangana State Agricultural University, Hyderabad, Telangana

4Senior Breeder, Rasi Seeds Pvt. Ltd. Tamilnadu, India

*Corresponding Author E-mail: msrkrishna_bt@kluniversity.in

Online published on 8 August, 2019.


Dragon fruit which belongs to Cacteceae family was once widely grown as ornamental plant and is now considered as fruit crop. Though there were many reports on propagating dragon fruit inadequate information is available on obtaining best quality pant material. Hence the present work is carried out to find the effect of phyto harmones, 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on callus formation, shoot and root regenerations from dragon fruit in vitro using stem cuttings as explants. Stem explants in Murashige Skoog (MS) medium containing 3mg/l BAP and 0.5mg/l NAA exhibited highest in vitro response for callus formation. Further, maximum number of shoots (13.2) was regenerated from explants when MS basal medium was supplemented with 3mg/l BAP and 0.5mg/l NAA. Within four weeks somatic shoots were raised from explants. It took 70 days for shoots to grow 2.0cm long. Subsequently regenerated shoots were transferred to rooting medium supplemented with 3mg/l BAP and 0.5mg/l NAA which proved to be effective concentrations for the growth and development of roots (4.8) in vitro.



Callus, Dragon fruit, MS basal medium, NAA, BAP, somatic shoots.


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